Researcher Information

SATO Takeo

Associate Professor

Molecular basis of plant stress response via ubiquitin system

Department of Biological Sciences, Cell Structure and Function


Molecular mechanism of plant adaptation to environmrntal stress via ubiquitin system

FieldPlant Physiology, Cell Biology, Biochemistry
KeywordSignal transduction, Ubiquitin modification, Proteomics, Environmental stress, High CO2, Flowering, Plant-microbe interaction

Introduction of Research

Ubiquitination is one of the fundamental post-translational modifications with ubiquitin, a conserved 76-amino acid protein present in eukaryotes. Ubiquitin forms several type of poly-ubiquitin chains to regulate protein degradation and subcellular localization, which plays critical roles in plant growth regulation in response to multiple environmental stresses. We previously identified a membrane localized ubiquitin ligase ATL31 regulating plant adaptation to carbon/nitrogen nutrient stress and defense response to pathogen attack. We are investigating biochemical and physiological significances of ubiquitin signals in plant stress adaptation.

Plant adaptation to environmental stressess (C/N nutrient availability and pathogen attack)
Ubiquitin system
Plant growth regulation by C/N nutrient signal
CO2/N affects plant biomass and senescence progression
Membrane localized ubiqutiin ligase ATL31 in Arabidopsis
BiFC analysis to examine in vivo protein-protein interaction

Representative Achievements

Deubiquitinating enzymes UBP12 and UBP13 limit stabilize the brassinosteroid receptor BRI1 (2022) Luo Y, Takagi J, Claus LAN, Zhang C, Yasuda S, Hasegawa Y, Yamaguchi J, Shan L, Russinova E and Sato T*, EMBO reports, 23: e53354.
The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis (2022) Hasegawa Y, Huarancca Reyes T, Uemura T, Baral A, Fujimaki A, Luo Y, Morita Y, Saeki Y, Maekawa S, Yasudaa S, Mukuta K, Fukao Y, Tanaka K, Nakano A, Takagi J, Bhalerao RP, Yamaguchi J and Sato T*, The Plant Cell, 34: 1354-1374.
Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis (2021) Sanagi M, Aoyama S, Kubo A, Lu Y, Sato Y, Ito S, Abe M, Mitsuda N, Ohme-Takagi M, Kiba T, Nakagami H, Rolland F, Yamaguchi J, Imaizumi T* and Sato T*, Proc. Natl. Acad. Sci. U. S. A., 118: e2022942118.
Protein phosphorylation dynamics under carbon/nitrogen-nutrient stress and identification of a cell death-related receptor-like kinase in Arabidopsis (2020) Li X, Sanagi M, -5-, Schulze WX, Regina F, Stitt M, Lunn JE, Nakagami H*, Sato T* and Yamaguchi J, Front. Plant Sci. 11: 377.
Arabidopsis CBL-Interacting Protein Kinases Regulate Carbon/Nitrogen-Nutrient Response by Phosphorylating Ubiquitin Ligase ATL31 (2017) Yasuda S, Aoyama S, Hasegawa Y, Sato T* and Yamaguchi J, Mol. Plant 10: 605-618.

Department of Biological Sciences, Cell Structure and Function

SATO Takeo

Associate Professor

What is your dream that you want to achieve through your research?

The ability to sense and respond to environmental stimuli is critical for the growth of all living organisms. Especially, plants have developed sophisticated mechanisms to robustly monitor and appropriately respond to the dynamic changes of environment stresses due to their immobility. We are conducting research to reveal the molecular mechanism of excellent environmental adaptation of plants.

What is the research theme that you are currently focusing on?

Flowering is the important phase transition from vegetative growth to reproductive growth, which is modulated by multiple environmental stimuli. We are investigating the integration mechanism of nutrient signal and flowering regulation.

Please tell us what you are proud of about your lab (staff, students, laboratory equipment, the number of papers published, etc.)

It’s a gathering of ambitious students! They also motivate me so much.