Researcher Information

SATO Takeo

Associate Professor

Molecular basis of plant stress response via ubiquitin system

Department of Biological Sciences, Cell Structure and Function


Molecular mechanism of plant adaptation to environmrntal stress via ubiquitin system

FieldPlant Physiology, Cell Biology, Biochemistry
KeywordSignal transduction, Ubiquitin modification, Proteomics, Environmental stress, High CO2, Plant-microbe interaction

Introduction of Research

Ubiquitination is one of the fundamental post-translational modifications with ubiquitin, a conserved 76-amino acid protein present in eukaryotes. Ubiquitin forms several type of poly-ubiquitin chains to regulate protein degradation and subcellular localization, which plays critical roles in plant growth regulation in response to multiple environmental stresses. We previously identified a membrane localized ubiquitin ligase ATL31 regulating plant adaptation to carbon/nitrogen nutrient stress and defense response to pathogen attack. We are investigating biochemical and physiological significances of ubiquitin signals in plant stress adaptation.

Plant adaptation to environmental stressess (C/N nutrient availability and pathogen attack)
Ubiquitin system
Plant growth regulation by C/N nutrient signal
CO2/N affects plant biomass and senescence progression
Membrane localized ubiqutiin ligase ATL31 in Arabidopsis
BiFC analysis to examine in vivo protein-protein interaction

Representative Achievements

Arabidopsis CBL-Interacting Protein Kinases Regulate Carbon/Nitrogen-Nutrient Response by Phosphorylating Ubiquitin Ligase ATL31 (2017) Yasuda S, Aoyama S, Hasegawa Y, Sato T* and Yamaguchi J, Mol. Plant 10: 605-618.
Direct Transcriptional Activation of BT Genes by NLP Transcription Factors Is a Key Component of the Nitrate Response in Arabidopsis (2017) Sato T, Maekawa S, Konishi M, Yoshioka M, Sasaki Y, Maeda H, Ishida T, Kato Y, Yamaguchi J and Yanagisawa S*, Biochem. Biophys. Res. Commun. 483:380-386.
Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.) (2016) Lu Y, Yasuda S, Li X, Fukao Y, Tohge T, Fernie AR, Matsukura C, Ezura H, Sato T* and Yamaguchi J, J. Proteomics 143: 254-264.
The C/N regulator ATL31 controls papilla formation in response to powdery mildew fungi penetration by interacting with SNARE SYP121 in Arabidopsis (2014) Maekawa S, Inada N, Yasuda S, Fukao Y, Fujiwara M, Sato T, Yamaguchi J, Plant Physiol. 164: 879-887.
Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis thaliana. (2011) Sato T, Maekawa S, Yasuda S, Domeki Y, Sueyoshi K, Fujiwara M, Fukao Y, Goto DB, Yamaguchi J, Plant J. 68: 137-146.