Researcher Information

ABE Kazuhiro


Understanding molecular mechanism of biological macromolecules at the chemical level, manupilate it, and creat it

Department of Chemistry, Organic and Biological Chemistry


Elucidating molecular mechanisms of membrane transport proteins including active tansporters

FieldBiochemistry, Structural biology, Molecular biology
KeywordX-ray crystallography, Cryo-EM, Primary transporters, Membrane proteins, P-type ATPases, Gastric proton pump, Phospholipid Flippase, Sodium pump, Lipid transport proteins

Introduction of Research

If thermal equilibrium means death, the asymmetric distribution of various substrates across the memebrane of the living cells is one of the impotant basis of the living system. This is created and maintaind by a number of active transporters including P-type ATPases, which mediate uphill transport a variety of substgrates, ranging from small cations to large phospholipids against their electrochemical gradient by utilizing ATP as an energy source. Employing biochemistry and structural biology, we try to elucidate how these protein molecules, which play a fundamental role in life, work at the "chemical" level.

Gastric proton pump acidifies our stomach to pH 1
Lipid flippase, which mediate phospholipid flipping, is involved in cellular apoptosis
Drug development with AI x Organic chemistry x Structural analysis

Representative Achievements

Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanism.Young, V.C., Nakanishi, H., Meyer, D.J., Nishizawa, T., Artigas, P & Abe, K. Nat. Commun., 13, 5270 (2022)
Gastric proton pump with two occluded K+ engineered with sodium pump-mimetic mutations. Abe, K., Yamamoto, K., Irie, K., Nishizawa, T. & Oshima, A. Nat. Commun., 12, 5709 (2021)
Transport cycle of plasma membrane flippase ATP11C by cryo-EM. Nakanishi, H., Nishizawa, T., Irie, K., Segawa, K., Nureki, O., Fujiyoshi, Y., Nagata, S. & *Abe, K. Cell Rep., 32, 108208 (2020)
A single K+-binding in the crystal structure of the gastric proton pump. Yamamoto, K., Dubey, V., Irie, K., Nakanishi, H., Khandelia, H., Fujiyoshi, Y. & Abe, K. eLife, 8, e47701 (2019)
Crystal structures of the gastric proton pump.Abe, K., Irie, K., Nakanishi, H., Suzuki, H. & Fujiyoshi, Y. Nature, 556, 214-218 (2018)

Related industries

Chemistry, Drug development, Medicine
Academic degreePh D
Self Introduction

From Sapporo. I am working on X-ray crystallography and cryo-EM single particle analysis to elucidate molecular mechanism of membrane transport proteins including gastric proton pump and other P-type ATPases. Big fun for beer in a microbrewery.

Academic background1999 B Sc, Faculty of Science, Hokkaido University
2001 M Sc, Graduate school of Science, Hokkaido University
2004 Ph D, Graduate School of Science, Hokkaido University
2003-2007 JSPS Research Fellow
2004- Posdoc, Graduate Shool of Science, Kyoto University
2012- Assistant Professor, Cellular and Structgural Physiology Institute, Nagoya University
2016- Associate Professor, Cellular and Structgural Physiology Institute, Nagoya University
2024- Professor, Faculty of Science, Hokkaido University
Affiliated academic societyThe Japanese Biochemical Society, The Biophysical Society of Japan, The Pharmaceutical Society of Japan
ProjectJST CREST, Cell Dynamics "Lipid scrambring system regulated by higher-order structure interaction"
Room addressScience Bulding 7 7-208