Research Note: Diethylstilbestrol reduces primordial germ cells in male Japanese quail
Abstract
This study investigated the detrimental effects of diethylstilbestrol (DES), an estrogenic endocrine-disrupting chemical, on the viability of primordial germ cells (PGCs), embryonic precursors of germ cells, in Japanese quail. We injected 50 or 100 nmol DES solubilized in sesame oil into the yolk of stage X embryos and assessed changes in the population and cell cycle properties of circulating PGCs in blood vessels and gonadal PGCs after 2.5- and 7-day incubations, respectively. Liquid chromatography tandem mass spectrometer and Western blotting analyses identified DEAD-box polypeptide 4 (DDX4) and proliferating cell nuclear antigen (PCNA) as a stem cell marker and proliferation marker of quail PGCs, respectively. Immunochemical analyses revealed……
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Article Information:
Shusei Mizushima, Asato Kuroiwa, Research Note: Diethylstilbestrol reduces primordial germ cells in male Japanese quail, Poultry Science, Volume 102, Issue 10, 2023
DOI: 10.1016/j.psj.2023.102910
