Two secured FACT recruitment mechanisms are essential for heterochromatin maintenance
FACT (facilitate chromatin transcription) is involved in heterochromatic silencing, but its mechanisms and function remain unclear. We reveal that the Spt16 recruitment mechanism operates in two distinct ways in heterochromatin. First, Pob3 mediates Spt16 recruitment onto the heterochromatin through its Spt16 dimerization and tandem PH domains. Without Pob3, Spt16 recruitment is partially reduced, exhibiting a silencing defect and impaired H2A/H2B organization. Second, …..
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Shinya Takahata, Saori Chida, Aoi Ohnuma, Motoyoshi Ando, Takahiro Asanuma, Yota Murakami,
Two secured FACT recruitment mechanisms are essential for heterochromatin maintenance,
Cell Reports, Volume 36, Issue 7, 2021, 109540, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2021.109540