Teachers

Stem cells and Reprogramming

YOSHIDA, IkuyaAssistant Professor

Reproductive and Developmental Biology
Field
Cytogenetics
Theme
Ikuya Yoshida studies many aspects of epigenetic regulation in mammalian stem cells during development.
Keyword
Mouse
X chromosome inactivation and reactivation
Reprogramming
Stem cell
Embryonal carcinoma cell

In somatic cells of female mammals, one of the two X chromosomes is inactivated and forms facultative heterochromatin that is stably maintained throughout somatic cell divisions. At the time of onset of meiosis, however, reversal of X chromosome inactivation occurs in female primordial germ cells. This phenomenon, X chromosome reactivation, can be recapitulated by distinct experimental procedures: (1) embryonic stem (ES) or embryonal carcinoma (EC) cell-fusion with female somatic cells, (2) induced pluripotent stem (iPS) cells derived from somatic cells by ectopic expression of several transcription factors, and (3) spontaneous X chromosome reactivation in a rare EC cell line that retained the inactive X chromosome. Using a combination of cell and molecular biology, we are studying the molecular basis of X chromosome reactivation in vivo and in vitro. We are particularly interested in the relationship between spontaneous X-reactivation and stochastic appearance of stem-like precursor cells in the EC cell culture.

References

  • Yoshida, I. (2002) Spontaneous reactivation of the inactive X chromosome in mouse embryonal carcinoma cells. Cytogenet. Genome Res., 99, 44-51.
Faculty

Faculty of Science
Department of Biological Sciences
Reproductive and Developmental Biology

Grad School

Graduate School of Life Science
Division of Life Science
Biosystems Science Course

Contact Information

Email: ikuya [atmark] sci.hokudai.ac.jp

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