Understanidng of avian spesific polyspermic fertilization system

MIZUSHIMA, ShuseiAssistant Professor

Reproductive and Developmental Biology
Avian Reproductive Biology
Molecular Mechanisms of fertilization and early development and establishment of novel gene modification methods in birds
Birds, Polyspermic fertilization
Egg activation
Genome editing

Birds exhibit physiologically polyspermic fertilization, in which the entry of several sperm into one egg is permitted. We have previously established avian intracytoplasmic sperm injection (ICSI) method and this technique have revealed that a single sperm is insufficient for triggering fertilization and subsequent early embryo development. In order to understand more comprehensive avian polyspermic fertilization, we try to elucidate the molecular mechanism which regulates sperm selection for zygotic formation, now.

We also try to establish a new method of avian transgenesis and gene editing by in vitro fertilization technique, because a progress in gene modification in birds has been hampered due to the lack of traditional strategies such as pronuclear injection of DNA, sperm vector and somatic nuclear transfer. Establishment of this new avian technologies will aid our understanding the mechanism of avian fertilization and sex differentiation and so on, as well as assisting in the production low allergenic egg via the disruption of allergen-related genes.


Domestic birds have the potential for efficient protein production from the oviduct into an egg, this physiological characteristic can be applied to avian biotechnology, which may become an efficient bioreactor producing valuable pharmaceutical proteins in eggs. If you have interested in our studies, you may come our laboratory.


  • Mizushima S, Hiyama G, Shiba K, Inaba K, Dohra H, Ono T, Shimada K,  Sasanami T. The birth of quail chicks after intracytoplasmic sperm injection. Development 2014;141:3799–3806.
  • Mizushima S. Establishment of intracytoplasmic sperm injection technique in Japanese quail and its possible application for poultry resources and transgenic birds. J. Poult. Sci. 2012; 49:225.

Kuroiwa Lab


Faculty of Science
Department of Biological Sciences
Reproductive and Developmental Biology

Grad School

Graduate School of Life Science
Division of Life Science
Biosystems Science Course

Contact Information

Faculty of Science, Building #5 5-1103
Email: smizus [atmark] sci.hokudai.ac.jp

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